Nitrosigine®
Also known as: Inositol-Stabilized Arginine Silicate, ASI, Arginine Silicate Inositol
Inositol-stabilized arginine silicate complex that provides sustained elevation of plasma arginine (up to 6 hours) and silicon, enhancing eNOS-mediated nitric oxide production, vascular compliance, and cognitive flexibility during physical exertion.
Last reviewed: 2024-08-01 · 4 indexed studies
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Overview
Nitrosigine® is a patented complex of bonded arginine silicate stabilized with inositol (ASI). It delivers a uniquely sustained and clinically superior elevation of plasma arginine and silicon levels compared to standard arginine, producing both vascular and cognitive performance benefits within a single 1500mg dose.
Mechanism & Function
Off-white inositol-stabilized arginine silicate powder. Fully water-soluble.
- Powder
- Capsule
- Tablet
- RTD
Powder is generally preferred for bioavailability or efficacy.
Mechanism of Action
The arginine-silicate bond creates a stable complex that resists gastric degradation, delivering intact arginine to systemic circulation for eNOS substrate availability. Silicon supports arterial wall elasticity and collagen synthesis in vascular endothelium. Inositol stabilizes the complex and independently supports cell signaling. The result is a sustained (1.5–6 hr) elevation in plasma arginine, nitrite/nitrate (NOx), and silicon — superior in duration to free-form arginine. Additionally, increased cerebral blood flow enhances cognitive processing speed and mental flexibility during exercise.
Physiological Role
Supplies bioavailable arginine for systemic NO production while co-delivering silicon for vascular structural support. The bonded complex provides pharmacokinetic advantages over free-form arginine with superior oral bioavailability and sustained release kinetics.
Primary Pathways
Performance / Health Relevance
Clinically shown to increase blood arginine levels within 30 minutes, peaking at 1.5 hours and remaining elevated for up to 6 hours. Enhances both peripheral vasodilation (muscle pump/blood flow) and cerebral blood flow (focus, reaction time). Effective at a single 1500mg dose — lower than typical arginine or citrulline doses.
Evidence Review
Practical Use & Dosage
- Minimum Effective Dose
- 750mg/day
- Optimal Range
- 1500mg/day (clinically validated dose)
- Upper / Caution Level
- 3000mg/day
- Timing
- 15–30 minutes pre-workout (rapid onset); effects sustained 5–6 hours
- Best Format
- Powder (mixes well in pre-workout formulas) or capsule
- Bioavailability
- Bonded arginine silicate resists gastric degradation. Plasma arginine increases significantly within 30 min and remains elevated up to 6 hours — superior duration to free-form arginine or AAKG. Single-dose efficacy at 1500mg (vs 6–8g required for L-citrulline).
- Absorption Considerations
- Can be taken on empty stomach or with food. No significant food interaction. The inositol stabilization prevents arginine degradation in the GI tract.
Safety & Interactions
Side Effects
- •Well tolerated in all clinical trials
- •No significant adverse events reported at recommended doses
Contraindications
- Hypotension (potent vasodilator)
Drug Interactions
- •PDE5 inhibitors (additive vasodilation — monitor blood pressure)
- •Nitrates (additive)
- •Antihypertensives (may potentiate blood pressure lowering)
Who Should Avoid
- •Those with clinically low blood pressure
- •Individuals on multiple vasodilatory medications
Monitoring
Monitor blood pressure if stacking with other vasodilators. Generally very well tolerated across all studied populations.
Frequently Asked Questions
Auto-generated from indexed evidence — dosage, safety, interactions, and more.
When is Nitrosigine® typically used or indicated?
Nitrosigine® is most commonly used for: Nitric oxide enhancement, Pre-workout vasodilation, Cognitive focus during training, Sustained blood flow. Supplies bioavailable arginine for systemic NO production while co-delivering silicon for vascular structural support. The bonded complex provides pharmacokinetic advantages over free-form arginine with superior oral bioavailability and sustained release kinetics.
How does Nitrosigine® work?
The arginine-silicate bond creates a stable complex that resists gastric degradation, delivering intact arginine to systemic circulation for eNOS substrate availability. Silicon supports arterial wall elasticity and collagen synthesis in vascular endothelium. Inositol stabilizes the complex and independently supports cell signaling. The result is a sustained (1.5–6 hr) elevation in plasma arginine, nitrite/nitrate (NOx), and silicon — superior in duration to free-form arginine. Additionally, increased cerebral blood flow enhances cognitive processing speed and mental flexibility during exercise.
What is the recommended dose of Nitrosigine®?
The optimal range is 1500mg/day (clinically validated dose). with a minimum effective dose of 750mg/day. Best taken: 15–30 minutes pre-workout (rapid onset); effects sustained 5–6 hours. Upper / caution level: 3000mg/day.
What are the side effects of Nitrosigine®?
Reported side effects include: Well tolerated in all clinical trials; and No significant adverse events reported at recommended doses. Monitoring: Monitor blood pressure if stacking with other vasodilators. Generally very well tolerated across all studied populations.
Does Nitrosigine® interact with medications?
Known or theoretical interactions: PDE5 inhibitors (additive vasodilation — monitor blood pressure); Nitrates (additive); and Antihypertensives (may potentiate blood pressure lowering). Always check with a clinician before combining with prescription medication.
Who should avoid Nitrosigine®?
Nitrosigine® is generally not recommended for: Those with clinically low blood pressure; Individuals on multiple vasodilatory medications; and Hypotension (potent vasodilator).
How strong is the evidence for Nitrosigine®?
Evidence level: Strong (overall score 82/100), based on 4 indexed studies on SuppIndex.
Research & References (4)
Inositol-stabilized arginine silicate increases blood arginine, nitric oxide, and vascular function
1500mg Nitrosigine significantly increased plasma arginine, silicon, and blood NOx levels within 30 minutes. Arginine levels remained elevated at 5 hours post-dose. Significant improvements in flow-mediated dilation (FMD) — a direct measure of vascular endothelial function.
Nitrosigine® supplementation improves cognitive function during acute exercise
Double-blind RCT showing 1500mg Nitrosigine improved cognitive flexibility (Trail Making Test), processing speed, and mental acuity during and after exercise, attributed to enhanced cerebral blood flow.
Effects of inositol-stabilized arginine silicate on post-exercise blood flow and muscle volume
1500mg Nitrosigine significantly increased pre- and post-exercise muscle blood volume and vasodilation compared to placebo. Effects persisted for the duration of the workout and post-exercise recovery period.
Safety assessment of inositol-stabilized arginine silicate (ASI; Nitrosigine®)
Comprehensive safety review across 30+ human studies confirming no clinically significant adverse events, no changes in blood chemistry panels, and GRAS self-determination for doses up to 1500mg/day.
Trademark Ingredients
Related Ingredients
Other top-rated ingredients in Pump and Performance Ingredients.
Beetroot Extract
80/100Concentrated source of dietary inorganic nitrate that undergoes serial reduction via oral microbiome (NO₃⁻ → NO₂⁻) and tissue-level reduction (NO₂⁻ → NO) through the oxygen-independent nitrate-nitrite-NO pathway, enhancing blood flow and exercise efficiency.
Citrulline Malate
78/100Salt of L-citrulline and DL-malic acid (commonly 2:1); citrulline raises plasma arginine and NO; malate enters the Krebs cycle and may buffer fatigue.
L-Citrulline
74/100A non-proteinogenic amino acid recycled through the urea cycle that elevates plasma L-arginine levels, enhancing endothelial nitric oxide synthase (eNOS) substrate availability and systemic NO production.
Research Overview
Strong EvidenceCite this ingredient
One-click citation export in APA, MLA, Chicago, BibTeX, or HTML. The URL below is stable and safe to link.
Stable URL
https://thesuppindex.com/ingredients/nitrosigine
SuppIndex. (2026). Nitrosigine: Evidence summary, dosage and clinical studies. Retrieved October 24, 2026, from https://thesuppindex.com/ingredients/nitrosigine
Based on 4 indexed studies · Evidence level: Strong.
Goal Ratings
Stack Compatibility
Stacks synergistically with L-citrulline for dual-pathway NO enhancement (citrulline → arginine recycling + direct ASI arginine delivery). Excellent with GlycerSize for volumizing pump effects. Compatible with all standard pre-workout ingredients.
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